In order to improve the reconstruction accuracy in fluorescence molecular tomography (FMT), a common approach is to increase the number of fluorescence data or projections. However, this approach consumes too much memory space and computational time.
AlPO4 mesoporous glass doped with perylene orange (PO) is prepared using two steps. The effects of dipping time and PO concentration on the fluorescent properties and molecular formation are investigated through absorption spectra, excitation spectra
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source. A high sensitivity cooled charge-coupled device (CCD) camera with excitation and emission filters is
Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measure
Fluorescence molecular tomography (FMT) allows the detection and quantification of various biological processes in small animals in vivo, which expands the horizons of pre-clinical research and drug development. Efficient three-dimensional (3D) recon
The Monte Carlo method is a versatile simulation algorithm to model the propagation of photons inside the biological tissues. It has been applied to the reconstruction of the fluorescence molecular tomography (FMT). However, such method suffers from
Fluorescence tomography can obtain a sufficient dataset and optimal three-dimensional images when projections are captured over 360? by CCD camera. Herein a non-stop dynamic sampling mode for fluorescence tomography is proposed in an attempt to impro
Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression, enzyme activity, and disease-specific molecular interactions in vivo using targeted contrast agents, and therefore, is being developed for
Even though multispectral imaging is considered very significant in biological imaging, it is only commonly used in microscopy in a 2D approach. Here, we present a Fluorescence Molecular Tomography system capable of recording simultaneously tomograph
Laminar optical tomography (LOT) is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography (DOT). Fluorescence LOT (FLOT) provides depth-resolved molecular information with 100-200μm resolution o
Challenges remain in imaging fast biological processes in vivo with fluorescence molecular tomography (FMT) due to the long data acquisition time. Data acquisition with limited projections can greatly reduce the time consumption, but the influence of
Recently, the simplified spherical harmonics equations (SPN) model has attracted much attention in modeling the light propagation in small tissue geometries at visible and near-infrared wavelengths. In this paper, we report an efficient numerical met
Fluorescence molecular tomography (FMT) is a fast-developing optical imaging modality that has great potential in early diagnosis of disease and drugs development. However, reconstruction algorithms have to address a highly ill-posed problem to fulfi
Optical barcodes have demonstrated a great potential in multiplexed bioassays and cell tracking for their distinctive spectral fingerprints. The vast majority of optical barcodes were designed to identify a specific target by fluorescence emission sp
Based on the generalized pulse spectrum technique that was previously developed for time-domain diffuse optical tomography, we propose a linear framework of time-domain fluorescence molecular tomography for simultaneous reconstruction of both the yie
Fluorescence spectra of acetic acid-water solution excited by ultraviolet (UV) light are studied, and the relationship between fluorescence spectra and molecular association of acetic acid is discussed. The results indicate that when the exciting lig
Accurate small animal surface reconstruction is important for full angle non-contact fluorescence molecular tomography (FMT) systems. In this letter, an optimal surface reconstruction method for FMT is proposed. The proposed method uses a line search
